Effect of Platelet-Rich Plasma on the Healing of Mandibular Defects Treated With Fresh Frozen Bone Allograft: A Radiographic Study in Dogs
The purpose of this study was to radiographically analyze the effect of autologous platelet rich plasma (PRP) on the healing of fresh frozen bone allograft (FFBA) placed in surgically created resection defects in mandibles of dogs. Bilateral resection defects measuring 1.5 cm × 1 cm were surgically created on the inferior border of the mandible in 10 adult male dogs. The defects were randomly divided into three groups: C, FFBA, and FFBA/PRP. In Group C, the defect was filled by blood clot only. In Group FFBA, the defect was filled with particulate fresh frozen bone allograft. In Group FFBA/PRP, it was filled with particulate fresh frozen bone allograft combined with PRP. At 90 days postoperative, standardized radiographs of the mandibles were obtained and results were quantitatively evaluated. Analysis of digitized radiographs indicated that non-PRP grafts were significantly less dense than the PRP grafts. Group FFBA/PRP also presented a statistically greater mineralized tissue area than Groups C and FFBA. Within the limits of this study, it can be concluded that PRP enhanced the healing of FFBA in resection defects in mandibles of dogs.
Figure 1. Surgical defect (1.5 cm × 1 cm) created on the inferior border of the mandible and protected with an absorbable surgical mesh. Figure 2. Computer-aided method for edge detection. (a) Digital radiographic image without Sobel filter; (b) Digital radiographic image with Sobel filter. The margins of the surgical defects (red line) are defined. Figure 3. The total area is delineated by the red line and corresponds to the area of the mandible where the surgical defect was originally created. The mineralized tissue area is delineated by the blue line.
Mean number of platelets per microliter (μl) and standard deviations in the samples of platelet rich plasma and whole blood.
Digital radiographic images of the surgical defects at 90 days postoperative. (a) Group C; (b) Group FFBA; and (c) Group FFBA/PRP.
Contributor Notes