Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 01 Jun 2011

The Effect of Interleukin-1 Allele 2 Genotype (IL-1a−889 and IL-1b+3954) on the Individual's Susceptibility to Peri-Implantitis: Case-Control Study

DDS and
DDS
Page Range: 325 – 334
DOI: 10.1563/AAID-JOI-D-09-00117.1
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Abstract

Individuals bearing the combination of interleukin (IL)-1 allele 2 at IL-1A−889 and IL-1B+3954 are referred to as being genotype positive and are susceptible to increased periodontal tissue destruction. The aim of this study was to assess the possible association of IL-1 allele 2 (IL-1A−889 and IL-B+3954) genotypes with the severity of peri-implantitis progression and the effect of this combination on treatment outcomes. Fifty patients with International Team for Implantology implants were studied; patients ranged in age from 35–55 years, and each patient had 1 implant. According to peri-implant tissue status, patients were divided into 2 groups: group I consisted of 25 patients with peri-implantitis, and group II comprised 25 patients with healthy peri-implant tissue. Clinical parameters were assessed at baseline and after 3 and 6 months. Epithelial cells were collected from the oral mucosa by plastic spatula and were used for IL-1 genotyping by the polymerase chain reaction technique. Group I patients were subjected to a peri-implantitis treatment and maintenance program. In all, 17 patients from group I and 5 patients from group II were genotype positive, with a statistically significant difference noted between the 2 groups. Group I genotype-positive patients presented with higher scores and measurements of clinical parameters with increased suppuration from peri-implant tissues compared with group II; differences were statistically significant (P < .05). In terms of response to treatment, genotype-negative patients demonstrated better response than genotype-positive patients. The combination of IL-1 allele 2 (IL-1A−889 and IL-1B+3954) in patients with inflamed periodontal or peri-implant tissues acts as a risk factor that leads to greater tissue destruction. IL-1 gene polymorphism at IL-1A−889 and IL-1B+3954 may affect outcomes of treatment for peri-implantitis in genotype-positive individuals.

Copyright: 2011 by the American College of Veterinary Internal Medicine
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F igure 1.

Polymerase chain reaction (PCR) assay result for interleukin (IL)-A−889 showing positive assay result at lanes 1, 3, 4, 5, and 6 at 99 base pairs. Lane 2 shows a negative PCR assay result. Lane B represents the reaction in the positive control.


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2.
F igure 2.

Polymerase chain reaction (PCR) assay result for interleukin (IL)-B+3954 showing positive assay result at lanes 1, 2, 4, and 5 at 194 base pairs. Lane 3 shows negative PCR assay result.


Contributor Notes

*Corresponding author, e-mail: hamdy.ahmed63@gmail.com
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