Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 01 Apr 2011

Evaluation of the Effect of Ozonated Plant Oils on the Quality of Osseointegration of Dental Implants Under the Influence of Cyclosporin A: An In Vivo Study

PhD, MS,
PhD, MSc,
MSc, MS, PhD,
BA, BDS, and
PhD
Page Range: 247 – 257
DOI: 10.1563/AAID-JOI-D-09-00098
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Abstract

Immunosuppressive agents have been recognized as factors that induce changes and modifications in bone metabolism. The purpose of this study was to evaluate the effect of ozonated plant extracts (herein termed ozonated oil) under the influence of Cyclosporin A (CsA) on osseointegration. A total of 20 dental implants were placed in 20 rabbit tibiae assigned to Group A or B. CsA was injected at an immunosuppressive dose in Groups A and B as a single-dose treatment. At the day of surgery, Group A received a single topical ozonated oil treatment (0.55 mL) around dental implants; Group B, the control group, received no ozonated oil. Animals were sacrificed after 8 weeks. Radiographs were obtained at implant surgery and on the day of sacrifice. Bone quality was compared between the 2 groups. Radiographically, osseointegration was microscopically evaluated using scanning electron and light microscopies. In ozonated Group A specimens, light microscopic examination demonstrated evidence of more organized mature bone compared with Group B. Within the limits of this study, the results suggest that short-term administration of CsA, when administered with topical ozonated oil, may influence bone density and the quality of dental implant osseointegration. Therefore, topically applied ozonated oil may influence bone density and the quality of osseointegration around dental implants.

Copyright: 2011 by the American College of Veterinary Internal Medicine
Figure 1
Figure 1

Radiograph of the tibia exhibiting the density line along the implant.


Figures 2 and 3
Figures 2 and 3

Figure 2. Histogram presenting the mean changes in density values of studied groups between T0 and T1. Figure 3. Histogram presenting the mean percent change in measurements at T0 and T1 in both studied groups.


Figures 4–7
Figures 4–7

Figure 4. Scanning electron microscopy for control group (Group B) showing bone contact along the implant surface. Figure 5. Scanning electron microscopy for ozonated group (Group A) showing intimate bone engagement along the irregular surface of the implant. Figure 6. ×10 ground section reveals bone implant interphase; the compact bone is mature, showing well-developed Haversian systems. Figure 7. ×10 ground section reveals bone implant interphase; the bone is less mature, with cellular osteoid tissue showing fibrous connective tissue and blood vessels.


Contributor Notes

*Corresponding author, e-mail: amhadary@netscape.net
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