Effect of Platelet-Rich Plasma on Peri-Implant Bone Repair: A Histologic Study in Dogs
The present study evaluated the effect of platelet-rich plasma (PRP) on peri-implant bone healing. A total of 9 mongrel dogs received 36 dental implants with sandblasted acid-etched surface in lower jaws in a split-mouth design: in the PRP group (n = 18 implants) the implants were placed in association with PRP, and in the control group (n = 18 implants) the implants were placed without PRP. Biopsies were obtained and prepared for histologic and histometric analysis after 15, 30, and 55 days of healing. The biopsies retrieved at 15 days showed delicate bone trabeculae formed by immature bone with presence of numerous osteoblasts for both groups. At 30 days the trabeculae presented reversal lines and evident lamellar disposition, where some thread spaces were filled by bone and dense connective tissue. At 55 days, bone healing was not altered in the control group, and histologic aspects were variable for the group treated with PRP. There was no significant difference between the groups for bone-to-implant contact (P > .05). PRP did not enhance bone formation around sandblasted acid-etched implants.Abstract
Assessment of bone to implant contact: red lines are bone to implant contact and green lines absence of bone contact.
Figure 2. Control group at 15 days. Longitudinal section. (a) Peri-implant spaces between threads are occupied by tissue resembling medullary tissue (empty arrow) or delicate bone trabeculae (dark arrow; hematoxylin and eosin [HE], original magnification [OM] ×40). (b and c) Peri-implant spaces filled with delicate bone trabeculae (dark arrow) and fibrous connective tissue (empty arrow; HE, OM ×100 and ×200, respectively). Figure 3. Treated group at 15 days. Longitudinal section. (a) Peri-implant spaces between threads are occupied by fibrous connective tissue (empty arrow) or delicate bone trabeculae (dark arrow; HE, OM ×40). (b and c) Peri-implant spaces filled with delicate bone trabeculae (dark arrow) and connective tissue resembling medullary tissue (empty arrow; HE, OM ×100 and ×200, respectively).
Figure 4. Control group at 30 days. Longitudinal section. (a) Peri-implant spaces between threads occupied by connective tissue similar to medullary tissue (empty arrow) or delicate bone trabeculae (dark arrow; hematoxylin and eosin [HE], original magnification [OM] ×40). (b and c) Peri-implant space occupied by bone trabeculae undergoing maturation (dark arrow; HE, OM ×100 and ×200, respectively). Figure 5. Treated group at 30 days. Longitudinal section. (a) Peri-implant spaces between threads occupied by delicate bone trabeculae (dark arrow; HE, OM ×40). (b and c) Lamellar bone and connective tissue similar to medullary tissue (empty arrow; HE, OM ×100 and ×200, respectively).
Figure 6. Control group at 55 days. Longitudinal section. (a) Peri-implant spaces between threads occupied by bone (dark arrow; hematoxylin and eosin [HE], original magnification [OM] ×40). (b and c) Peri-implant spaces occupied by bone (dark arrow) presenting areas partially filled with amorphous material resembling bone (empty arrow; HE, OM ×100 and ×200, respectively). Figure 7. Treated group at 55 days. Longitudinal section. (a) Peri-implant spaces between threads occupied by bone (dark arrow; HE, OM ×40). (b and c) Thin bone trabeculae in the peri-implant spaces (dark arrow) and connective tissue similar to medullary tissue (empty arrow; HE, OM ×100 and ×200, respectively).
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