Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 01 Jun 2010

Effects of Platelet-Rich Plasma and Chitosan Combination on Bone Regeneration in Experimental Rabbit Cranial Defects

DDS, PhD,
MD,
DDS, PhD,
PhD,
DDS, PhD,
DDS, PhD, MS, and
DDS, PhD
Page Range: 175 – 184
DOI: 10.1563/AAID-JOI-D-09-00023
Save
Download PDF

Abstract

This study aimed to investigate and compare the effect of chitosan sponge and platelet-rich plasma (PRP) gel alone as well as their combination on bone regeneration in rabbit cranial defects. Four cranial defects with a 4.5-mm diameter were created in rabbit cranium and grafted with PRP, chitosan sponge alone, and chitosan sponge incorporated with PRP. The rabbits were killed by the fourth and eighth weeks, and the defects were analyzed histologically. Higher bone formation was observed in the PRP group when compared with the other groups at weeks 4 and 8. All parts of the defects were filled with thick trabecular new bone in the PRP group. The amount of new bone formation in the control groups was found to be less when compared with the PRP group and the least in the chitosan group. The defects that were filled with chitosan sponge showed a limited amount of new bone formation and an obvious fibrous demarcation line between chitosan particles and bone. Application of PRP showed a histological tendency toward increased bone formation. Other forms or derivatives of chitosan may have beneficial effects to achieve new bone regeneration.

Copyright: 2010 by the American College of Veterinary Internal Medicine
Figure 1
Figure 1

(a) Rabbit cranium with surgical sites prepared. (b) Rabbit cranium with surgical sites grafted.


Figure 2
Figure 2

Histologic evaluation of all grafted sites at 4 weeks (magnification, ×10). (a) Control defect that was not grafted. (b) Platelet-rich plasma (PRP). (c) Chitosan. (d) PRP + chitosan combination. NB indicates new bone; BM, bone marrow; IR, inflammatory reaction; C, chitosan.


Figure 3
Figure 3

Histologic evaluation of all grafted sites at 8 weeks (magnification, ×10). (a) Control defect that was not grafted. (b) Platelet-rich plasma (PRP). (c) Chitosan. (d) PRP + chitosan combination. NB indicates new bone; BM, bone marrow; IR, inflammatory reaction; C, chitosan; FC, fibrous capsulation.


Contributor Notes

Faculty of Dentistry, Department of Periodontology, Hacettepe University, Sihhiye, Ankara, Turkey.
Associate professor, Gülhane Military Medical Academy, Department of Orthopedics and Traumatology, Etlik, Ankara, Turkey.
*Corresponding author, e-mail: burakdemiralp@yahoo.com
Associate professor, Hacettepe University, Faculty of Dentistry, Department of Periodontology, Sihhiye, Ankara, Turkey.
Professor, Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Sihhiye, Ankara, Turkey.
Professor, Hacettepe University, Faculty of Dentistry, Department of Periodontology, Sihhiye, Ankara, Turkey.
  • Download PDF