Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 01 Apr 2008

Influence of Matrix-Suspended Demineralized Bone on Osseous Repair Using a Critical-Sized Defect in the Rat (Rattus norvegicus) Calvarium

DDS, MS,
DDS, PhD,
DMD, MS,
DMD,
DDS, MS,
DDS, MS,
PhD, and
PhD
Page Range: 83 – 89
DOI: 10.1563/1548-1336(2008)34[83:IOMDBO]2.0.CO;2
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Abstract

Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100% DFDB, 47% DFDB/polyol, 47% DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100% DFDB group exhibited the most bone fill. The 47% DFDB/polyol and 47% DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100% DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.

Copyright: American Academy of Implant Dentistry
Figure 1.
Figure 1.

Decalcified hematoxylin and eosin histology of the critical-sized defects: (a) control, (b) polyol, (c) 47% DFDB group, (d) 47% DFDB/polyol, and (e) 100% DFDB.


Figure 2.
Figure 2.

Scanning electron microscope view (×20 magnification) 254 × 190 mm (96 × 96 dpi) of demineralized bone particles: (a) 100% DFDB, (b) 47% DFDB, and (c) 47% DFDB/polyol.


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