Avoiding Leukocyte Contamination and Early Platelet Activation in Platelet-Rich Plasma
The objective of this study was to describe a new platelet-rich plasma (PRP) protocol with a reduced concentration of leukocytes and intact platelets. We collected 8 mL of venous blood (VB) from marginal ear veins of 10 male New Zealand white rabbits in acid dextrose citrate Vacutainer tubes. Tubes were centrifuged at 302g for 10 minutes. All plasma was collected in plastic tubes to avoid buffy-coat contamination and centrifuged at 2862g for 5 minutes. A 10% calcium chloride activator (10 PRP:2 CaCl2) was added to the lower third of this plasma (PRP), and the PRP gel was obtained. Mean platelet count was 317.7 × 103 ± 39.9/μL in VB and 1344.9 × 103 ± 347.5/μL in PRP. Leukocyte counts were 3.96 × 103 ± 2.01/μL and 0.46 × 103 ± 0.45/μL in VB and PRP, respectively. Mean platelet enrichment was 327.4 ± 97.8%. All differences were statistically significant (P > .05). This protocol is practical and reproducible, resulting in a high concentration of intact platelets to help tissue repair and low levels of leukocytes.Abstract
Contributor Notes
Ivy K. Trindade-Suedam, DDS, MS, Fábio R. M. Leite, DDS, MS, and Juliana A. N. D. de Morais, DDS, MS, are with the Department of Diagnostic and Surgery, Division of Periodontics, School of Dentistry at Araraquara, São Paulo State University-UNESP. Address correspondence to Dr Ivy Kiemle Trindade-Suedam at Rua Dr. Antônio Prudente 4-141, Jardim Estoril II, Bauru – SP, Brazil, CEP 17016-010 (ivytrin@uol.com.br).
Elza R. M. Leite, MS, is with the Immunohematology Division, Araraquara Blood Bank, São Paulo State University-UNESP.
Elcio Marcantonio, Jr, DDS, MS, PhD, is an assistant professor, Department of Diagnostic and Surgery, Division of Periodontics, School of Dentistry at Araraquara, São Paulo State University-UNESP.
Amauri A. Leite, MS, PhD, is an assistant professor, Department of Clinical Analysis, School of Pharmacy at Araraquara – UNESP.