Screw vs Cement-Implant–retained Restorations: An Experimental Study in the Beagle. Part 2. Immunohistochemical Evaluation of the Peri-implant Tissues
Crestal bone loss has been reported to occur around dental implants. Even if the causes of this bone loss are not completely understood, the presence of a microgap between implant and abutment with a possible contamination of the internal portion of the implants has been suggested. The aim of this study was to see if there were differences in the vascular endothelial growth factor (VEGF) expression, microvessel density (MVD), proliferative activity (MIB-1), and inflammatory infiltrate in the soft tissues around implants with screwed and cemented abutments. Sandblasted and acid-etched implants were inserted in the mandibles of 6 Beagle dogs. Ten 3.5- × 10-mm root-form implants were inserted in each mandible. A total of 60 implants (30 with screwed abutments and 30 with cemented abutments) were used. After 12 months, all the bridges were removed and all abutments were checked for mobility. A total of 8 loosened screws (27%) were found in the screwed abutments, whereas no loosening was observed in cemented abutments. A gingival biopsy was performed in 8 implants with cemented abutments, in 8 implants with screwed abutments, and in 8 implants with unscrewed abutments. No statistically significant differences were found in the inflammatory infiltrate and in the MIB-1 among the different groups. No statistically significant difference was found in the MVD between screwed and cemented abutments (P = .2111), whereas there was a statistically significant difference in MVD between screwed and unscrewed abutments (P = .0277) and between cemented and unscrewed abutments (P = .0431). A low intensity of VEGF was prevalent in screwed and in cemented abutments, whereas a high intensity of VEGF was prevalent in unscrewed abutments. These facts could be explained by the effects induced, in the abutments that underwent a screw loosening, by the presence of bacteria inside the hollow portion of the implants or by enhanced reparative processes.Abstract
Contributor Notes
Bartolomeo Assenza, MD, DDS, is a visiting professor, Dental School, University of Chieti-Pescara, Chieti, Italy, and is in private practice in Cologno Monzese (MI), Italy.
Antonio Scarano, DDS, MD, is a researcher, Vittoria Perrotti, DDS, is a research fellow, Maurizio Piattelli, MD, DDS, is an associate professor, Marcello Piccirilli, BSc, is a chief technician, and Adriano Piattelli, MD, DDS, is a professor of Oral Pathology and Medicine, Dental School, University of Chieti-Pescara, Chieti, Italy. Address correspondence to Prof Adriano Piattelli at Via F. Sciucchi 63, 66100 Chieti, Italy (apiattelli@unich.it).
Luciano Artese, MD, is a professor of Pathology, University of Chieti-Pescara, Chieti, Italy.
Corrado Rubini, MD, is a researcher in the Department of Pathologic Anatomy and Histopathology, University of Ancona, Ancona, Italy.
Ulf Thams, MD, DDS, is in private practice in Madrid, Spain.
Fidel San Roman, MD, is a professor of Surgery, Director of Veterinary Teaching Hospital, Complutense University, Madrid, Spain.