Effect of Enamel Matrix Derivative (Emdogain) on Bone Defects in Rabbit Tibias
The aim of this study was to assess the effect of an enamel matrix derivative (Emdogain, Biora, AB, Malmö, Sweden) on bone healing. Ten New Zealand rabbits, weighing about 2.5 kg, were used. One 8-mm bone defect was created in each tibia. The defect on the right leg was filled with Emdogain, whereas the defect on the opposite leg was left unfilled as control. A total of 20 defects were created. Five rabbits each were killed at 4 and 8 weeks with an overdose of Tanax. Block sections containing the defects were retrieved and the specimens processed for light microscopy examination. The slides were stained with acid and basic fuchsin and toluidine blue. Histologically, no differences were noted in both groups at each observation period; in the test group, remnants of the implanted Emdogain were not present at 4 weeks. Newly formed bone was detectable in both groups at all observation times. At 8 weeks, both groups showed mature bone, and in the test group the material implanted was not visible. No inflammatory cells were visible in both groups. In conclusion, our results indicate that Emdogain implanted in bone defects is fully resorbed after 4 to 8 weeks and does not adversely affect bone formation.Abstract
Contributor Notes
Roberto Cornelini, MD, DDS, is a visiting professor in the Dental School, University of Chieti, Chieti, Italy, and is in private practice in Rimini, Italy.
Antonio Scarano, DDS, is a research fellow and Maurizio Piattelli, MD, DDS, is an associate professor in the Dental School, University of Chieti, Chieti, Italy.
Sebastiano Andreana, DDS, MS, is a clinical assistant professor, Department of Periodontology and Endodontics School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY.
Ugo Covani, MD, DDS, is an associate professor in the Dental School, University of Genova, Genova, Italy.
Alessandro Quaranta, DDS, is a research fellow in the Dental School, University of Tor Vergata, Rome, Italy.
Adriano Piattelli, MD, DDS, is a professor of Oral Pathology and Medicine in the Dental School, University of Chieti, Chieti, Italy. Address correspondence to Professor Adriano Piattelli at F. Sciucchi 63, 66100 Chieti, Italy.